human coronary artery smooth muscle cells hcasmcs  (Cell Applications Inc)

Journal: Advanced Materials (Deerfield Beach, Fla.)

Article Title: Deployable 3D‐Printed Vascular Stent with Surface‐Catalysed Endogenous Nitric Oxide Generation

doi: 10.1002/adma.202520199

Figure Lengend Snippet: In vitro biocompatibility and intracellular NO generation characterisation of DSENO materials. (a, b) Cell viability of HUVECs (a) and HCASMCs (b) after 48 h incubation with printed DSENO plates. (c) Quantitative analysis of material‐induced intracellular NO generation. Endogenous NO production in HUVECs was quantified by measuring the mean fluorescence intensity (MFI) of the DAF‐FM probe using high‐resolution confocal laser scanning microscopy (CLSM). (d) CLSM images of HUVECs after 48 h incubation with material candidates, cells stained with DAF‐FM (green) and Hoechst (blue) ( n ≥ 6). Scale bar = 100 µm. Data are normalized to the blank control and represent the average MFI of multiple regions of interest. Error bars indicate standard deviation; statistical significance was determined via one‐way ANOVA ( * p < 0.05).

Article Snippet: Human coronary artery smooth muscle cells (HCASMCs) from Cell Applications were maintained in human smooth muscle cell growth medium (311–500) and passaged at 70–90% confluency.

Techniques: In Vitro, Incubation, Fluorescence, Confocal Laser Scanning Microscopy, Staining, Control, Standard Deviation

Journal: JACC: Basic to Translational Science

Article Title: HDL Regulates TGFβ-Receptor Lipid Raft Partitioning, Restoring Contractile Features of Cholesterol-Loaded Vascular Smooth Muscle Cells

doi: 10.1016/j.jacbts.2025.101461

Figure Lengend Snippet: Contractile Gene Expression Is Downregulated in Chol-Loaded hVSMCs (A, B) Human vascular smooth muscle cells (hVSMCs) were treated with cholesterol (Chol) (5 μg/mL) or 0.2% bovine serum albumin (control [CT]) for 24 hours and 48 hours and gene expression of Acta2 , Tagln , Cnn1, Myocd, and Srf were determined by quantitative polymerase chain reaction. (C) hVSMCs were treated with Chol (5 μg/mL) or 0.2% bovine serum albumin (CT) for 24 hours and protein expression of α–smooth muscle actin (α-SMA) and CNN1 were determined by Western blotting (representative blots shown). Densitometry showing the (D) α-SMA and (E) CNN1 band intensities normalized to GAPDH. For data analysis, unpaired Student’s t -testing was performed for comparing the means of 2 groups. For 2 or more independent groups, 1-way analysis of variance followed by Dunnett post hoc test was performed. A P value of ≤0.05 was considered significant. Data are presented as the mean ± SEM of 3 independent experiments, and P values are as indicated (∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001).

Article Snippet: Human coronary artery smooth muscle cells (referred to as hVSMC) were purchased from Cell Applications and maintained in complete medium (#311-500) as provided by the vendor. hVSMCs were used within 8 passages for all experiments.

Techniques: Gene Expression, Control, Real-time Polymerase Chain Reaction, Expressing, Western Blot